Introduction to Next Generation Sequencing Analysis
Overview
Instructor(s): Dr. Michael Dickens
Time: Friday, September 19, 2025 — 1:30PM-4:00PM CT
Location: Blocker 202
Prerequisite(s): Active HPRC account, basic Linux/Unix skills
This short course covers concepts of sequence mapping, variant calling and ChIP-seq analysis utilizing tools available on the HPRC clusters.
A Registration button will appear here when registration has been opened.
Course Materials
Learning Objectives and Agenda
In this course, participants will learn:
- An overview of NGS Technology
- How to find and use Template Job Scripts
- Sequence Quality Control (QC)
- How to Visualize BAM Alignment Files
- Fundamentals of Sequence Variant Calling
- How to use BioContainers
This course focuses, among others, on the following topics:
- HPRC Cluster Utilities
- NGS Technology
- NGS Tools on Grace
- Template Job Scripts
- Quality Control (QC)
- Visualize BAM Alignment Files
- Sequence Variant Calling
- BioContainers
Note: During the class sessions many aspects of the material will be illustrated live via a login to Grace. Attendees will follow along and complete the exercises on their own laptops.
You are encouraged to contact the HPRC helpdesk with any questions regarding Grace.