Overview

Instructor(s): Dr. Wesley Brashear

Time: Friday, September 26, 2025 1:30PM-4:00PM CT

Location: Blocker 220

Prerequisite(s): Active HPRC account

This short course introduces concepts of metagenomics analysis based on data generated by Next Generation Sequencing (NGS) technology.

Course Materials

Presentation slides

The presentation slides are available as downloadable PDF files.

• NGS Metagenomics (Fall 2022): PDF

Previous Semester Materials

• NGS Metagenomics (Fall 2019): PDF
• NGS Metagenomics commands (Fall 2019): TXT
• NGS Metagenomics job script (Fall 2019): SH
• NGS Metagenomics (Spring 2019): PDF
• NGS Metagenomics commands (Spring 2019): PDF
• NGS Metagenomics (Fall 2018): PDF
• NGS Metagenomics commands (Fall 2018): txt
• NGS Metagenomics (Spring 2018): PDF
• NGS Metagenomics commands (Spring 2018): txt
• NGS Metagenomics (Fall 2017): PDF

Agenda

In this course, participants will:

• Learn the different sequencing strategies employed in metagenomics
• Learn how to complete a full single-gene marker metagenomics workflow using the QIIME2 software suite

This course focuses, among others, on the following topics:

• Sequencing strategies
• QIIME2
• Data preprocessing
• OTU clustering
• Alpha diversity analysis
• Beta diversity analysis

Note: During the class sessions many aspects of the material will be illustrated live via a login to an HPRC Cluster. Attendees will log into the Grace cluster using their own computers to follow along and complete exercises.

You are encouraged to contact the HPRC helpdesk with any questions regarding HPRC clusters.