NGS Metagenomics
Overview
Instructor(s): Dr. Wesley Brashear
Time: Friday, September 26, 2025 1:30PM-4:00PM CT
Location: Blocker 220
Prerequisite(s): Active HPRC account
This short course introduces concepts of metagenomics analysis based on data generated by Next Generation Sequencing (NGS) technology.
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Course Materials
Presentation slides
The presentation slides are available as downloadable PDF files.
- NGS Metagenomics (Fall 2025): PDF
Previous Semester Materials
Agenda
In this course, participants will:
- Learn the different sequencing strategies employed in metagenomics
- Learn how to complete a full single-gene marker metagenomics workflow using the QIIME2 software suite
This course focuses, among others, on the following topics:
- Sequencing strategies
- QIIME2
- Data preprocessing
- OTU clustering
- Alpha diversity analysis
- Beta diversity analysis
Note: During the class sessions many aspects of the material will be illustrated live via a login to an HPRC Cluster. Attendees will log into the Grace cluster using their own computers to follow along and complete exercises.
You are encouraged to contact the HPRC helpdesk with any questions regarding HPRC clusters.