Overview

Instructor(s): Dr. Wesley Brashear

Time: Friday, February 13, 2026 1:30PM-4:00PM CT

Location: Blocker 220

Prerequisite(s): Active HPRC account

This short course introduces concepts of metagenomics analysis based on data generated by Next Generation Sequencing (NGS) technology.

Course Materials

• NGS Metagenomics (Fall 2025): PDF

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• NGS Metagenomics (Fall 2022): PDF

Participation

During the training, attendees are expected to use their own computer and complete the instructor-led examples and exercises.

Learning Objectives and Agenda

In this course, participants will:

• Learn the different sequencing strategies employed in metagenomics
• Learn how to complete a full single-gene marker metagenomics workflow using the QIIME2 software suite

This course focuses, among others, on the following topics:

• Sequencing strategies
• QIIME2
• Data preprocessing
• OTU clustering
• Alpha diversity analysis
• Beta diversity analysis