ACES: NGS Metagenomics
Overview
Instructor(s): Dr. Wesley Brashear
Time: Tuesday, April 15, 2025 — 1:30PM-4:00PM CT
Location: online using Zoom
Prerequisite(s): Current ACCESS ID, basic Linux/Unix skills, and basic familiarity with NGS techniques/terminology
This short course introduces concepts of metagenomics analyses based on data generated by Next Generation Sequencing (NGS) technology using the ACES cluster.
Course Materials
Presentation slides
The presentation slides are available as downloadable PDF files.
- NGS Metagenomics (Spring 2025): PDF
Previous Semester Materials
Learning Objectives and Agenda
In this course, participants will:
- Learn the different sequencing strategies employed in metagenomics
- Learn how to complete a full single-gene marker metagenomics workflow using the QIIME2 software suite
This course focuses, among others, on the following topics:
- Sequencing strategies
- QIIME2
- Data preprocessing
- OTU clustering
- Alpha diversity analysis
- Beta diversity analysis
Note: During the class sessions many aspects of the material will be illustrated live via a login to FASTER/ACES. Attendees will log into the ACES cluster using their own computers to follow along and complete exercises.
You are encouraged to contact the HPRC helpdesk with any questions regarding FASTER/ACES.