ACES: NGS Metagenomics

Overview

Instructor: Wesley Brashear

Time: Tuesday, November 5, 2024 — 10:00AM-12:30PM CT

Location: online using Zoom

Prerequisites: Current ACCESS ID, basic Linux/Unix skills, and basic familiarity with NGS techniques/terminology

This short course introduces concepts of metagenomics analysis based on data generated by Next Generation Sequencing (NGS) technology using the ACES cluster.

Registration will open up on this webpage the week before the class.

Course Materials

Presentation slides

The presentation slides are available as downloadable PDF files.

  • NGS Metagenomics (Fall 2024): PDF

Previous Semester Materials

  • NGS Metagenomics (Spring 2024): PDF
  • NGS Metagenomics (Fall 2023): PDF
  • NGS Metagenomics (Spring 2023): PDF

Learning Objectives and Agenda

In this course, participants will:

  • Learn the different sequencing strategies employed in metagenomics
  • Learn how to complete a full single-gene marker metagenomics workflow using the QIIME2 software suite

This course focuses, among others, on the following topics:

  • Sequencing strategies
  • QIIME2
  • Data preprocessing
  • OTU clustering
  • Alpha diversity analysis
  • Beta diversity analysis

Note: During the class sessions many aspects of the material will be illustrated live via a login to FASTER/ACES. Attendees will log into the ACES cluster using their own computers to follow along and complete exercises.

You are encouraged to contact the HPRC helpdesk with any questions regarding FASTER/ACES.