ACES: NGS Metagenomics


Instructor: Wesley Brashear

Time: Tuesday, April 9, 2024 — 10:00AM-12:30PM CT

Location: online using Zoom

Prerequisites: Current ACCESS ID, basic Linux/Unix skills, and basic familiarity with NGS techniques/terminology

This short course introduces concepts of metagenomics analysis based on data generated by Next Generation Sequencing (NGS) technology using the ACES cluster.

Course Materials

Presentation slides

The presentation slides are available as downloadable PDF files.

  • NGS Metagenomics (Spring 2024): PDF

Previous Semester Materials

  • NGS Metagenomics (Fall 2023): PDF
  • NGS Metagenomics (Spring 2023): PDF


This course focuses, among others, on the following topics:

  • Sequencing strategies
  • QIIME2
  • Data preprocessing
  • OTU clustering
  • Alpha diversity analysis
  • Beta diversity analysis

Note: During the class sessions many aspects of the material will be illustrated live via a login to FASTER/ACES. Attendees will log into the ACES cluster to follow along with these parts using their own computers.

You are encouraged to contact the HPRC helpdesk with any questions regarding FASTER/ACES.