ACES: NGS Metagenomics
Overview
Instructor(s): Dr. Wesley Brashear
Time: Tuesday, April 15, 2025 — 1:30PM-4:00PM CT
Location: online using Zoom
Prerequisite(s): Current ACCESS ID, basic Linux/Unix skills, and basic familiarity with NGS techniques/terminology
This short course introduces concepts of metagenomics analyses based on data generated by Next Generation Sequencing (NGS) technology using the ACES cluster.
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Course Materials
Presentation slides
The presentation slides are available as downloadable PDF files.
- NGS Metagenomics (Spring 2025): PDF
Previous Semester Materials
Learning Objectives and Agenda
In this course, participants will:
- Learn the different sequencing strategies employed in metagenomics
- Learn how to complete a full single-gene marker metagenomics workflow using the QIIME2 software suite
This course focuses, among others, on the following topics:
- Sequencing strategies
- QIIME2
- Data preprocessing
- OTU clustering
- Alpha diversity analysis
- Beta diversity analysis
Note: During the class sessions many aspects of the material will be illustrated live via a login to FASTER/ACES. Attendees will log into the ACES cluster using their own computers to follow along and complete exercises.
You are encouraged to contact the HPRC helpdesk with any questions regarding FASTER/ACES.