Overview

Instructor: Wesley Brashear

Time: Friday, March 22, 2024 — 10:00AM-12:30PM CT

Location: Blocker 220

Prerequisites: Current HPRC account

This short course covers concepts of RNA-seq data analysis for differential expression, including Quality Control, reference-based read alignment, utilization of DESeq2, and data visualization in R.

Course Materials

• RNA-seq and Differential Expression (Spring 2024): PDF

Previous Semester Materials

• RNA-seq and Differential Expression (Fall 2022): PDF

Agenda

This course focuses, among others, on the following topics:

• Generating RNA-seq libraries
• Experimental design
• Transcriptome assembly overview
• Quality Control/Library trimming
• Aligning RNA-seq libraries to a reference genome
• Generating count files
• Differential expression in R
• Small RNA analysis