NGS RNA Sequencing


Instructor: Shichen Wang

Time: Friday July 31 10:30AM-1:00PM

Location: Zoom session only

Prerequisites: Ada and Terra account

This short course covers concepts of RNA-seq data analysis for Transcriptome assembly, differential expression and variant calling.

Course Materials

Presentation slides

The presentation slides are available as downloadable PDF files.

  • Introduction to NGS (Spring 2020): PDF
  • Introduction to NGS (Fall 2017): PDF


This course focuses, among others, on the following topics:

NGS RNA Sequencing

  • Reference Genomes
  • Quality Control (QC)
  • Aligning Sequences to a Reference Genome
  • Transcriptome Assembly
  • Transcriptome Evaluation and Annotation
  • Differential Expression (DE)
  • Variant Calling

Note: During the class sessions many aspects of the material will be illustrated live via a login to Ada. Attendees are welcome to follow these parts with their own laptops. They will need to configure their laptops to use the TAMULink wireless network. Relevant details on this can be found at:

You are encouraged to contact the HPRC helpdesk with any questions regarding Ada.